Lysis with qiagen protease or proteinase k qiaamp dna blood mini kits contain qiagen protease. These products are not intended for the diagnosis, prevention, or treatment of a disease. Qiaamp dna mini and blood mini handbook moodle ufsc. In accordance with qiagens isocertified quality management system, each lot of the rneasy mini kit, rneasy protect mini kit, and rneasy plant mini kit is tested against predetermined specifications to. Dna extracts obtained by the qiaamp dna stool mini kit yield a higher amount of dna compared to dna extracts obtained by easymag from the same fecal samples. Qiaamp midi and maxi spin columns can also be processed using the qiavac 24 plus. The qiaamp dna mini kit provides silicamembranebased nucleic acid purification from. After dislodging the buccal cells by vortexing in pbs, they are lysed in. The purelink viral rnadna mini kit provides a rapid and efficient method to simultaneously purify viral rnadna from fresh or frozen cellfree biological fluids plasma, serum, cerebrospinal fluid and cell. In this study, we used human blood, buffy coat and a total of 76 simulated touch dna samples to test the effectiveness of the following five common dna extraction methods, namely, chelex 100, qiaamp dna blood mini kit, qiaamp dna investigator kit, qiasymphony dna investigator kit and dna iq system, in the recovery of such dna. High yield, high quality genomic dna from cattle nasal. Qiaamp dna mini and blood mini handbook 112007 7 product use limitations the qiaamp dna mini kit and qiaamp dna blood mini kit are intended for general laboratory use.
Evaluation of qiaamp dna stool mini kit for ecological. Supp prot saliva dna qiagen qiaamp blood mini kit v1. Qiagen provides a variety of qiaamp genomic dna kits for fast, efficient and easy dna. Qiaamp dna stool mini kit was purchased from qiagen canada and used according to the manufacturers instructions. Third edition june 2012 qiaamp dna mini and blood mini handbook for dna purification. Dna extraction protocol for iswabdna using qiaamp dna mini. The qiaamp dna mini kit and qiaamp dna blood mini kit are intended for molecular biology applications. No claim or representation is intended for their use to. The purelink viral mini kit is specifically designed to isolate highq. Performagene dna genotek, ottawa is a dna collection kit which uses nasal samples resulting in an easytouse, quick and reliable sampling method that provides high quantity, high. Qiagen qiaamp cador pathogen mini kit, 250 preps, 50 to 150. Qiagen qiaamp dna mini kit, 250 preps, 200l sample, 50 to 200l elution volume, whole. For isolation of genomic, mitochondrial, bacterial, parasite or viral dna. The qiaamp dna mini kit provides silicamembranebased nucleic acid purification from tissues, swabs, csf.
Qiaamp dna stool mini kit handbook 082001 qiaampdna stool procedure qiaamp dna stool mini kit handbook 082001 10 purification qiaampspin columns qiaampdna purification. Purelink viral rnadna mini kit thermo fisher scientific. Laboratory protocol for dna extraction from oracollectdx ocd. Qiaamp dna mini kit and qiaamp dna blood mini kit handbook. The qiaamp dna mini kit provides silicamembranebased nucleic acid purification from tissues, swabs, csf, blood, body fluids, or washed ce. Join the qiagen cancer research community get exclusive access to our first.
Qiaamp genomic dna kits 122015 7 qiaamp dna micro kit purification of genomic and mitochondrial dna from small samples the qiaamp dna micro kit is designed for purification of genomic, mitochondrial and viral dna from small amounts of fresh or frozen blood, tissue and dried blood spots. For isolation of viral rna and dna and bacterial dna from a variety of animal sample types. Qiaamp viral rna mini handbook 122007 5 product use limitations the qiaamp viral rna mini kit is intended for general laboratory use. Qiagen qiaamp dna mini kit, 50 preps, 200l sample, 50 to 200l elution volume, whole blood, tissue, cells sample, silica technology, spin column format, manual processing, 20 min. Qiaamp rna blood mini kit 50 52304 from qiagen sample to. These kits use the same silicamembrane technology as the qiaamp dna blood mini kit. The qiaamp dna stool mini kit provides silica membranebased purification of up to 30. To set up and operate the qiavac 24 plus for a particular application, refer to. Dneasy plant handbook 102012 7 introduction dneasy plant kits provide a fast and easy way to purify dna from plant and fungal tissue. Qiaamp cador pathogen mini kit 250 from qiagen selectscience. Qiagen qiaamp dna mini kit, 50 preps, 200l sample, 50 to 200l elution volume, whole. Dna isolation with qiagen qiaamp dna mini kit this protocol applies to.
Qiaamp genomic dna kits 122015 7 qiaamp dna micro kit purification of genomic and mitochondrial dna from small samples the qiaamp dna micro kit is designed for purification of genomic. This study evaluated the qiagen qiaamp dna mini kit and the schleicher and schuell isocode stix dna isolation device for isolating nucleic acid. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. Briefly, bacterial samples were homogenized in buffer asl and heated at 95 c for 5 min to lyse bacterial cells. Buffer, serum, and wholeblood samples were spiked with. Qiaamp minelute ccfdna mini kit 55204 exorneasy maxi kit 77164 available in united states. Efficient, rapid, and reproducible procedures for isolating highquality dna before pcr gene amplification are essential for the diagnostic and molecular identification of pathogenic bacteria. Do not add qiagen protease or proteinase k directly to buffer al. Qiaamp dna micro handbook 082003 7 introduction the qiaamp dna micro kit uses wellestablished technology for purification of genomic and mitochondrial dna from small sample volumes or sizes. Qiaamp dna mini kit and qiaamp dna blood mini kit 092001 7 product use limitations qiaamp kits are intended as generalpurpose devices. In this study, a semiautomatic dna extraction system easymag, biomerieux, marcy ietoile, france and a manual one qiaamp dna stool mini kit, qiagen, hilden, germany were tested on stool samples collected from 3 patients with inflammatory bowel disease ibd and 5 healthy individuals.
Acute lymphoblastic leukemia phase i all p1, acute lymphoblastic leukemia phase 2 all p2, and acute lymphoblastic. Timerun, 4 to 12g yield, rapid purification of highquality, readytouse dna, ideal for pcr, southern. Qiagen qiaamp rna blood mini kit, 50 preps, 50 to 1. When using the qiaamp dna blood mini kit for a sample that requires a modified protocol. Microbial diversity in fecal samples depends on dna. The flexigene dna kit provides scalable purification of genomic dna. Lyophilized qiagen protease can be stored at room temperature 1525c for up to 6 months without any decrease in performance. The qiaamp dna mini kit yields dna sized up to 50 kb see figure purification of up to 50 kb genomic dna. Qiaamp dna mini kit nucleic acid purification bacterial. Briefly, bacterial samples were homogenized in buffer asl and heated at. Dna purified using the qiaamp dna mini kit can be used in a wide range of downstream applications, including pcr and quantitative realtime pcr, southern blotting, snp and str genotyping, and pharmocogenomic research. Qiaamp dna mini kit bacterial or genomic dna purification from water, filters, or surface swabs by qiagen.
Intensive research has shown that qiagen protease is the optimal enzyme for use with the lysis buffer. The qiaamp dna mini kit provides silicamembranebased nucleic acid purification from tissues, swabs, csf, blood, body fluids, or washed cells from urine. We recommend all users of qiagen products to adhere to the nih guidelines. Qiaamp dna stool mini kit handbook 082001 qiaampdna stool procedure qiaamp dna stool mini kit handbook 082001 10 purification qiaampspin columns qiaampdna purification procedure involves digestion proteins,binding dna qiaampsilicagel membrane, washing away impurities, puredna from spincolumn. The purelink viral rnadna mini kit provides a rapid and efficient method to simultaneously purify viral rnadna from fresh or frozen cellfree biological fluids plasma, serum, cerebrospinal fluid and cell culture supernatants. Third edition june 2012 qiaamp dna mini and blood mini handbook for dna purification from. Qiaamp dna mini kit and qiaamp dna blood mini kit 022003 7 product use limitations qiaamp kits are intended as generalpurpose devices. Up to 100 mg of tissue can be processed using the dneasy plant. Laboratory protocol for dna extraction from oracollectdx. Supplementary protocol for dna isolation from saliva preserved with norgens saliva dna collection and preservation device using qiagen qiaamp dna blood mini kit components required component. The following risk and safety phrases apply to components of the rneasy mini kit, the rneasy protect mini kit, andor the rneasy plant mini kit. The kit combines the selective binding properties of a silicabased membrane with flexible elution volumes of between 20 and 100 l. An evaluation of the performance of five extraction methods.
For purification of cellular rna from fresh whole blood. No claim or representation is intended to provide information for. Jul 20, 2015 this tutorial explains how to carry out a miniprep using the qiaprep spin miniprep kit provided by qiagen. Qiagen qiaamp dna mini kit, 50 preps, 200l sample, 50 to 200l elution volume.
View qiaamp dna mini kit gdna extraction kit manual. There is no consensus over the method to be used precisely for microbial dna, can qiagen kit for blood and tissue or dnarna dual kit can be used. Dna purified using the qiaamp dna mini kit can be used in a wide range of downstream applications, including pcr and quantitative realtime pcr, southern blotting, snp and str genotyping, and. Furthermore, dna extracts obtained using easymag seemed to contain inhibitory compounds, since in order to perform a successful pcranalysis, the sample should be diluted at least 10. No claim or representation is intended for their use to identify any specific organism or for clinical use diagnostic, prognostic, therapeutic, or blood banking. This protocol is designed for purification of total dna from grampositive bacteria. Refer to the handbook for protocols for swabs, dried blood spots, cultured cells, fixed tissue, bacteria, yeast or other material. With a qiagen qiaamp mini kit bloomsburg university of. Up to 100 mg of tissue can be processed using the dneasy plant mini kit or up to 1 g of tissue using the dneasy plant maxi kit. Human buccal cell dna isolation with a qiagen qiaamp mini kit i.
A small quantity human cheek buccal cells are obtained with an oral swab with a soft bristled, sterile cytobrush. In accordance with qiagens isocertified quality management system, each lot of the rneasy mini kit, rneasy protect mini kit, and rneasy plant mini kit is tested against predetermined specifications to ensure consistent product quality. The kit combines the selective binding properties of a silicabased membrane with flexible elution volumes of between 20 and 150 l. Dna adsorbs to the dneasy membrane in the presence of high concentrations. There is no consensus over the method to be used precisely for microbial dna, can qiagen kit for blood and tissue or dna rna dual kit can be used. Promega, easymag biomerieux and qiaamp blood dna kit qiagen for extracting dna from whole blood sample. Qiaamp dna blood mini kits, or qiagen products in general, please do not hesitate to contact us.